OPTIMUM DOSES OF DEUTERIUM-OXIDE AND SODIUM-BROMIDE FOR THE DETERMINATION OF TOTAL-BODY WATER AND EXTRACELLULAR FLUID

A practical approach for determining optimum tracer doses is described for measurements of total body water (TBW) and extracellular water (ECW) based on dilution of deuterium oxide and sodium bromide with respective analyses by nuclear magnetic resonance and anion-exchange chromatography. Using these techniques and plasma concentrations corresponding to adult doses up to 1.5 g kg-1 body weight of deuterium oxide and 0.05 g kg-1 of sodium bromide, the variations of analyses of these tracers, at these respective doses, were calculated. TBW determination with an RSD of less than 2% was found to require administration of 0.4 g kg-1 of deuterium oxide. Because basal concentrations of bromide are quantifiable, the accuracy of the extracellular water determination depends upon the magnitude of the increase in plasma bromide concentration; a sodium bromide dose of 0.01 g kg-1 provides a deviation in the determined ECW volume of approximately 1%.