APPLICATION OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY AND INDIRECT UV DETECTION FOR THE ANALYSIS OF FATTY-ACIDS

被引：56
|
作者：
ERIM, FB
XU, X
KRAAK, JC
机构：
[1] Laboratory for Analytical Chemistry, University of Amsterdam, 1018 WV Amsterdam
来源：
JOURNAL OF CHROMATOGRAPHY A | 1995年 / 694卷 / 02期
关键词：
D O I：
10.1016/0021-9673(94)01114-T
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C-8-C-20 fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.
引用
收藏
页码：471 / 479
页数：9
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