OPTIMIZATION OF THE DETECTION WAVELENGTH APPLIED TO THE HPLC ANALYSIS OF POLYCYCLIC AROMATIC-HYDROCARBONS

Presented paper discuss a mathematical procedure which utilises the individual spectra of fifteen polyaromatic hydrocarbons for the optimization of the detection wavelength. Procedure is based upon use of weighting factors appropriate for the components under consideration (toxicological data). Two criteria have been used in the optimization procedure. If suggested criterion was used and all the components were of equal importance, then the optimum was at 220 and 254 nm respectively. When the weighting factors based on the toxicological data were employed, the optimum was found at 287 nm.