OCCURRENCE IN LEGUMINOUS SEEDS, RESISTANCE TO PROTEASE DIGESTION AND ANTIGENICITY OF AN ALPHA-AMYLASE INHIBITOR

被引:4
|
作者
KOTARU, M [1 ]
IWAMI, K [1 ]
YEH, HY [1 ]
IBUKI, F [1 ]
机构
[1] KYOTO PREFECTURAL UNIV,DEPT AGR CHEM,KYOTO 606,JAPAN
关键词
D O I
10.1016/0308-8146(92)90240-3
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A rabbit was immunized with the purified alpha-amylase inhibitor from cranberry beans to obtain a specific antiserum. With respect to antigenicity, the inhibitor was examined for its distribution in leguminous seeds, and was proved to occur exclusively in cultivars of the same kind (Phaseolus vulgaris) but not in those of other kinds. IN addition, its in-vitro digestibility was investigated by the use of porcine and bovine enzymes. Consequently it was revealed that the inhibitor underwent a gradual inactivation by chymotrypsin digestion (but not by pepsin and trypsin digestions) and that its antigenicity was removed more slowly than its inhibitor activity.
引用
下载
收藏
页码:47 / 50
页数:4
相关论文
共 50 条
  • [1] FURTHER CHARACTERIZATION OF THE 12 KDA PROTEASE ALPHA-AMYLASE INHIBITOR PRESENT IN MAIZE SEEDS
    BLANCOLABRA, A
    CHAGOLLALOPEZ, A
    MARTINEZGALLARDO, N
    VALDESRODRIGUEZ, S
    JOURNAL OF FOOD BIOCHEMISTRY, 1995, 19 (01) : 27 - 41
  • [2] ALPHA-AMYLASE INHIBITOR LEVELS IN SEEDS GENERALLY AVAILABLE IN EUROPE
    GRANT, G
    EDWARDS, JE
    PUSZTAI, A
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1995, 67 (02) : 235 - 238
  • [3] ALPHA-AMYLASE INHIBITOR IN BARLEY-SEEDS - LOCALIZATION AND QUANTIFICATION
    LECOMMANDEUR, D
    LAURIERE, C
    DAUSSANT, J
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 1987, 25 (06) : 711 - 715
  • [4] ALPHA-AMYLASE INHIBITOR, NOT PHYTOHEMAGGLUTININ, EXPLAINS RESISTANCE OF COMMON BEAN-SEEDS TO COWPEA WEEVIL
    HUESING, JE
    SHADE, RE
    CHRISPEELS, MJ
    MURDOCK, LL
    PLANT PHYSIOLOGY, 1991, 96 (03) : 993 - 996
  • [5] ARGININE IS ESSENTIAL FOR THE ALPHA-AMYLASE INHIBITORY ACTIVITY OF THE ALPHA-AMYLASE/SUBTILISIN INHIBITOR (BASI) FROM BARLEY-SEEDS
    ABE, J
    SIDENIUS, U
    SVENSSON, B
    BIOCHEMICAL JOURNAL, 1993, 293 : 151 - 155
  • [6] INHIBITION OF RYE ALPHA-AMYLASE ACTIVITY BY BARLEY ALPHA-AMYLASE INHIBITOR
    TORRONEN, A
    LEISOLA, M
    HAARASILTA, S
    CEREAL CHEMISTRY, 1992, 69 (04) : 355 - 358
  • [7] VARIATION IN ALPHA-AMYLASE AND ALPHA-AMYLASE INHIBITOR ACTIVITIES IN BARLEY MALTS
    HENSON, CA
    STONE, JM
    JOURNAL OF CEREAL SCIENCE, 1988, 8 (01) : 39 - 46
  • [8] ALPHA-AMYLASE INHIBITORS IN PLANT SEEDS
    SHIVARAJ, B
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 1977, 14 (01): : 54 - 54
  • [9] Structural characterization of an alpha-amylase inhibitor from a wild common bean (Phaseolus vulgaris): Insight into the common structural features of leguminous alpha-amylase inhibitors
    Nakaguchi, T
    Arakawa, T
    Philo, JS
    Wen, J
    Ishimoto, M
    Yamaguchi, H
    JOURNAL OF BIOCHEMISTRY, 1997, 121 (02): : 350 - 354
  • [10] OCCURRENCE OF ALPHA-AMYLASE AND BETA-AMYLASE IN PROPOLIS
    KACZMAREK, F
    DEBOWSKI, WJ
    ACTA POLONIAE PHARMACEUTICA, 1983, 40 (01): : 121 - 121