Growth stimulation of CHO cells by phospholipids in serum-free culture

被引：0
|
作者：
Sakai, K
Matsunaga, T
Yamaji, H
Fukuda, H
机构：
[1] Kobe Univ, Grad Sch Sci & Technol, Fac Engn, Dept Sci & Chem Engn, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Grad Sch Sci & Technol, Div Mol Sci, Kobe, Hyogo 6578501, Japan
来源：
ANIMAL CELL TECHNOLOGY: CHALLENGES FOR THE 21ST CENTURY | 1999年
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effects of phosphatidic acid (PA) and lysophosphatidic acid (LPA) on the growth of Chinese hamster ovary (CHO) cells in serum-free media were evaluated by MTT assay. Supplementing PA or LPA to a protein-free basal synthetic medium or a serum-free medium containing insulin and transferrin markedly stimulated the growth of CHO cells, suggesting that PA and LPA are promising growth-promoting supplements for use in constituting a low-protein serum-free medium.
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页码：63 / 67
页数：5
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