DETERMINATION OF THE AMINO-ACID-SEQUENCE OF CYSTINE-CONTAINING PEPTIDES BY TANDEM MASS-SPECTROMETRY

被引：8

作者：

DESPEYROUX, D ^{[1
]}

BORDASNAGY, J ^{[1
]}

JENNINGS, KR ^{[1
]}

机构：

[1] UNIV WARWICK,DEPT CHEM,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1991年 / 5卷 / 04期

关键词：

D O I：

10.1002/rcm.1290050404

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the determination of the amino-acid sequence of a cyclic peptide containing cystine. It is based on the reduction of the peptide in a reductive matrix prior to ionization by fast-atom bombardment. The amino-acid sequence of the resulting linear peptide is then determined by tandem mass spectrometry from the spectrum produced by the collision-induced decomposition of the [M + H]+ ion of the peptide.

引用

页码：156 / 159

页数：4

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