BOVINE BRONCHIAL EPITHELIAL-CELLS METABOLIZE L-ARGININE TO L-CITRULLINE - POSSIBLE ROLE OF NITRIC-OXIDE SYNTHASE

被引：51

作者：

ROBBINS, RA ^{[1
]}

HAMEL, FG ^{[1
]}

FLOREANI, AA ^{[1
]}

GOSSMAN, GL ^{[1
]}

NELSON, KJ ^{[1
]}

BELENKY, S ^{[1
]}

RUBINSTEIN, I ^{[1
]}

机构：

[1] UNIV NEBRASKA,MED CTR,DEPT INTERNAL MED,PULM & CRIT CARE MED SECT,OMAHA,NE 68198

来源：

LIFE SCIENCES | 1993年 / 52卷 / 08期

关键词：

D O I：

10.1016/0024-3205(93)90232-R

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The conversion of L-arginine to L-citrulline is catalyzed by nitric oxide synthase (NOS), and results in the release of nitric oxide (NO). We hypothesized that bronchial epithelial cells metabolize L-arginine to L-citrulline. We found that cell lysates obtained from unstimulated, cultured bovine bronchial epithelial cells (BBECs) converted L-[H-3]arginine to L-[H-3]citrulline. This conversion was attenuated by three competitive NOS inhibitors and modulated by lipopolysaccharide and cigarette smoke extract (p<0.01, all comparisons) . These data demonstrate that BBECs metabolize L-arginine to L-citrulline and implicate a role for the L-arginine:NOS biosynthetic pathway in modulating airway responses.

引用

页码：709 / 716

页数：8

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