CHARACTERIZATION OF A REPLICATION OF THE MODERATELY PROMISCUOUS PLASMID, PGSH5000, WITH FEATURES OF BOTH THE MINI-REPLICON OF PCU1 AND THE ORI-2 OF F

被引：0

作者：

DASILVATATLEY, FM ^{[1
]}

STEYN, LM ^{[1
]}

机构：

[1] UNIV CAPE TOWN,SCH MED,DEPT MED MICROBIOL,CAPE TOWN 7925,SOUTH AFRICA

来源：

MOLECULAR MICROBIOLOGY | 1993年 / 7卷 / 05期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dominant, polA1-independent replicon of PGSH500, repbeta (1.8kb), consists of a cis-acting oriV region of 245bp; a repB gene that is essential for autonomous replication and 18, 30 to 36bp iterons which constitute the inc/cop region. The molecular organization of repbeta resembles that of mini-pCU1 (IncN). Furthermore, there is a 58% identity between the Rep proteins of these replicons. RepB also shows a 31% identity with RepE of mini-F. In addition, an 80% identity over 200bp was identified between the cis-acting beta oriV region and the equivalent region of ori-2 (mini-F). Replicons with deletions of repB could be complemented by Rep (pCU1) and RepE (mini-F) in trans, supporting the hypothesis that repbeta is a natural hybrid between a pCU1-like and F-like replicon.

引用

页码：805 / 823

页数：19