HETEROLOGOUS INVITRO TRANSCRIPTION FROM 2 ARCHAEBACTERIAL PROMOTERS

被引：9

作者：

HUDEPOHL, U ^{[1
]}

GROPP, F ^{[1
]}

HORNE, M ^{[1
]}

ZILLIG, W ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT BIOCHEM,SAN FRANCISCO,CA 94143

来源：

FEBS LETTERS | 1991年 / 285卷 / 02期

关键词：

TRANSCRIPTION INITIATION; CELL-FREE EXTRACT; PROMOTER; EVOLUTION; PHAGE-PHI-H; GAS VACUOLE GENE;

D O I：

10.1016/0014-5793(91)80811-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cell-free extract of Sulfolobus shibatae is able to specifically initiate transcription in vitro at the promoter of the plasmid-encoded gene for the major gas vesicle protein of Halobacterium halobium and at the promoter for the transcript T4 of the temperature H. halobium phage PHI-H. The corresponding promoter from the virulent phage mutant PHI-HL1 yields enhanced transcription in the heterologous system, in agreement with strongly increased in vivo expression.

引用

页码：257 / 259

页数：3

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