COMPARISON OF DIFFERENT STAINING METHODS FOR POLYVINYLIDENE DIFLUORIDE MEMBRANES

被引：21
|
作者：
CHRISTIANSEN, J [1 ]
HOUEN, G [1 ]
机构：
[1] UNIV COPENHAGEN,INST BIOCHEM GENET,OSTER FARIMAGSGADE 2A,DK-1353 COPENHAGEN,DENMARK
来源：
ELECTROPHORESIS | 1992年 / 13卷 / 03期
关键词：
D O I：
10.1002/elps.1150130137
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Several new staining methods for polyvinylidene difluoride membranes, including mercurochrome, silver and dimethylaminoazobenzene isothiocyanate staining were compared with Coomassie Brilliant Blue and gold staining. Of these, Coomassie was most versatile and completely compatible with ensuing microsequencing, immunostaining or other visualization methods, while gold and silver staining were more sensitive. Mercurochrome allows selective detection of sulf-hydryl-containing proteins while dimethylaminoazobenzene isothiocyanate staining may allow quantitation of sequenceable protein.
引用
收藏
页码：179 / 183
页数：5
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