Role of 6 integrin subunit in branching morphogenesis of fetal mouse submandibular gland : investigation by mesenchyme-free epithelial culture system

被引：10
|
作者：
Koyama, Noriko [1 ]
Hayashi, Toru [1 ]
Gresik, Edward W. [2 ]
Kashimata, Masanori [1 ]
机构：
[1] Asahi Univ, Sch Dent, Dept Pharmacol, 1851 Hozumi, Mizuho, Gifu 5010296, Japan
[2] CUNY, Sch Med, Dept Cell Biol & Anat, New York, NY 10031 USA
来源：
JOURNAL OF MEDICAL INVESTIGATION | 2009年 / 56卷
关键词：
alpha; 6; integrin; Branching morphogenesis; Cleft formation; Stalk elongation; Submandibular gland;
D O I：
10.2152/jmi.56.247
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The submandibular gland (SMG) of the fetal mouse is a well-studied model for the epithelial-mesenchymal interactions required for branching morphogenesis (BrM), which involves cleft formation and stalk elongation. In a previous report, we showed that alpha 6 integrin subunit is involved in BrM of this gland rudiment, since the neutralizing antibody against alpha 6 integrin subunit, GoH3, strongly inhibited branching of cultured intact E13 SMG. In this study, we investigated whether GoH3 inhibits cleft formation and/or stalk elongation during BrM of cultured mesenchyme-free epithelial rudiments of fetal SMG and also analyzed by Western blotting the levels of phosphorylation of ERK1/2, which is a signaling molecule known to regulate BrM of the fetal mouse SMG.
引用
收藏
页码：247 / 249
页数：3
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