FIBER-OPTIC-BASED IMMUNOSENSORS FOR HAPTENS

被引：27
|
作者：
BETTS, TA
CATENA, GC
HUANG, JF
LITWILER, KS
ZHANG, J
ZAGROBELNY, J
BRIGHT, FV
机构：
[1] Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14214, Acheson Hall
来源：
ANALYTICA CHIMICA ACTA | 1991年 / 246卷 / 01期
基金：
美国国家科学基金会;
关键词：
FLUOROMETRY; FIBEROPTIC SENSOR; IMMUNOSENSORS; HAPTENS;
D O I：
10.1016/S0003-2670(00)80664-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A selective fiber-optic-based immunosensor for haptens is described and characterized. In order to construct a sensor for different analytes, a prescribed reaction protocol is followed and only the recognition element [F(ab') antibody fragment] has to be changed. The dynamic range of this sensor is about two orders of magnitude and the detection limits are at least 25 nM (25-mu-l volume). By using steady-state fluorescence and classical ultra-high-vacuum surface analytical techniques, the effects of regeneration media, reaction and storage times and interferents on the sensor response were studied.
引用
收藏
页码：55 / 63
页数：9
相关论文
共 50 条
  • [1] RECENT ADVANCES IN REGENERABLE FIBER-OPTIC-BASED IMMUNOSENSORS
    BRIGHT, FV
    LITWILER, KS
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1990, 199 : 69 - ENVR
  • [2] FIBER-OPTIC-BASED FLUOROIMMUNOSENSORS
    SEPANIAK, MJ
    VODINH, T
    [J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 1990, 333 (1628): : 85 - 92
  • [3] Fiber-optic-based interferometric sensor
    Ayoub, Ahmad B.
    Nader, Abd El-Rahman
    Saad, Mai
    Gan, Qiaoqiang
    Swillam, Mohamed
    [J]. PHOTONIC INSTRUMENTATION ENGINEERING IV, 2017, 10110
  • [4] REGENERABLE FIBER-OPTIC-BASED IMMUNOSENSOR
    BRIGHT, FV
    BETTS, TA
    LITWILER, KS
    [J]. ANALYTICAL CHEMISTRY, 1990, 62 (10) : 1065 - 1069
  • [5] FIBER-OPTIC-BASED BIOCATALYTIC BIOSENSORS
    ARNOLD, MA
    [J]. ACS SYMPOSIUM SERIES, 1989, 403 : 303 - 317
  • [6] NEW FIBER-OPTIC-BASED SENSORS IN CHEMISTRY
    HIEFTJE, GM
    BRIGHT, FV
    BRUSHWYLER, KR
    CARROLL, MK
    CHILDERS, AG
    FREEMAN, JE
    MONNIG, CA
    POIRIER, GE
    VICKERS, GH
    WYATT, WA
    [J]. JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 1987, 134 (8B) : C524 - C524
  • [7] Multipoint fiber-optic-based corrosion sensor
    Martins-Filho, Joaquim F.
    Fontana, Eduardo
    Guimaraes, J.
    Coelho, I. J. Souza
    [J]. 19TH INTERNATIONAL CONFERENCE ON OPTICAL FIBRE SENSORS, PTS 1 AND 2, 2008, 7004
  • [8] A fiber-optic-based programmable delay line
    不详
    [J]. MICROWAVE JOURNAL, 1999, 42 (07) : 176 - +
  • [9] FIBER OPTIC IMMUNOSENSORS
    SLOVACEK, RE
    BLUESTEIN, BI
    [J]. JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 1987, 134 (8B) : C524 - C524
  • [10] MULTIDIMENSIONAL FLUORESCENCE WITH A SIMPLE FIBER-OPTIC-BASED PROBE
    LITWILER, KS
    BRIGHT, FV
    [J]. ACS SYMPOSIUM SERIES, 1989, 403 : 380 - 395
12345