PROLONGED CELL-FREE PROTEIN-SYNTHESIS IN A BATCH SYSTEM USING WHEAT-GERM EXTRACT

被引：27

作者：

KAWARASAKI, Y ^{[1
]}

NAKANO, H ^{[1
]}

YAMANE, T ^{[1
]}

机构：

[1] NAGOYA UNIV, SCH AGR,DEPT APPL BIOL SCI,MOLEC BIOTECHNOL LAB, CHIKUSA KU, NAGOYA, AICHI 46401, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 10期

关键词：

D O I：

10.1271/bbb.58.1911

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reaction conditions of cell-free protein synthesis using wheat germ extract were examined to prolong the period of protein synthesis in a batch reaction. By optimizing conditions for ATP regeneration system involved in the cell-free system, protein synthesis continued about 4 hours, so that about 17 mu g dihydrofolate reductase protein was obtained in 1 ml of a reaction mixture. It suggests that maintaining ATP concentration is the primary requirement for long-life cell-free protein synthesis.

引用

页码：1911 / 1913

页数：3

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