SEPARATION OF GARDENIA YELLOW COMPONENTS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

被引：0
|
作者：
OKA, H
IKAI, Y
YAMADA, S
HAYAKAWA, J
HARADA, KI
SUZUKI, M
NAKAZAWA, H
ITO, Y
机构：
[1] MEIJO UNIV, FAC PHARM, TENPAKU KU, NAGOYA, AICHI 468, JAPAN
[2] NATL INST PUBL HLTH, MINATO KU, TOKYO 108, JAPAN
[3] NHLBI, BIOPHYS CHEM LAB, BETHESDA, MD 20892 USA
来源：
MODERN COUNTERCURRENT CHROMATOGRAPHY | 1995年 / 593卷
关键词：
D O I：
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中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
High-speed countercurrent chromatography (HSCCC) has been successfully applied to preparative separation of gardenia yellow components. Three components, geniposide, trans-crocin, and 13-cis-crocin, were isolated from 25mg of the sample using a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:3:5). Due to the exposure to uv light emitted from the fluorescent room lamp, trans-crocin and 13-cis-crocin were photoisomerized to 13-cis- and trans-forms, respectively. It is also the first time that the presence of 13-cis-crocin and two glycosyl esters of crocetin in gardenia yellow is demonstrated by frit fast atom bombardment liquid chromatography/mass spectrometry and HPLC with photodiode array detection.
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页码：92 / 106
页数：15
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