LOCALIZATION OF GABA(A) RECEPTORS IN THE RABBIT RETINA

The distribution of gamma-aminobutyric acid, (GABA(A)) receptors in the rabbit retina is investigated and compared with the distribution of GABAergic neurons using immunocytochemical methods. Antibodies against the alpha 1, beta 2/3, and gamma 2 subunits of the GABA(A) receptor label subpopulations of bipolar, amacrine and ganglion cells. Double labeling experiments show that the gamma 2 subunit is colocalized with the alpha 1 and the beta 2/3 subunits in bipolar, amacrine and ganglion cells. Electron microscopy reveals that in the outer plexiform layer, GABA(A) receptor immunoreactivity is present on dendrites of cone bipolar cells adjacent to the cone pedicles. Bipolar cell dendrites are also receptor-positive at synapses from interplexiform cells. Some receptor immunoreactivity is found intracellularly in processes of horizontal cells. In the inner plexiform layer, GABA(A) receptor immunoreactivity is present on both rod bipolar and cone bipolar axon terminals at putative GABAergic input sites. Amacrine and ganglion cell processes in sublamina a and b are also labeled.