BINDING OF THE LIPID-PEROXIDATION PRODUCT 4-HYDROXYNONENAL TO HUMAN POLYMORPHONUCLEAR LEUKOCYTES

4-Hydroxynonenal (HNE) is produced during peroxidation of polyunsaturated fatty acids. It exerts a chemokinetic effect on human polymorphonuclear leukocytes (PMN). Investigations of this mechanism were performed. The results indicate that [H-3]-HNE binding to PMN results both in non-specific bonds to the numerous SH groups of the cells and in binding to a saturable, reversible and specific HNE site. Scatchard analysis revealed that this is a single site with an apparent affinity constant of 319 nM and a density of 1.57 pmol (10(6))(-1) cells. This specific binding site may be involved in the chemokinetic effect of HNE.