LOCALIZATION OF METHANOL DEHYDROGENASE IN 2 STRAINS OF METHYLOTROPHIC BACTERIA DETECTED BY IMMUNOGOLD LABELING

被引：21
|
作者：
FASSEL, TA
BUCHHOLZ, LA
COLLINS, MLP
REMSEN, CC
机构：
[1] UNIV WISCONSIN,DEPT BIOL SCI,MILWAUKEE,WI 53201
[2] UNIV WISCONSIN,CTR GREAT LAKE STUDIES,MILWAUKEE,WI 53201
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 07期
关键词：
D O I：
10.1128/AEM.58.7.2302-2307.1992
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of the methylotroph Methylobacterium sp. strain AM1 and to the intracytoplasmic membrane of the methanotroph Methylomonas sp. strain A4. Antibody cross-reactivity to other methylotrophic bacteria was detected.
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收藏
页码：2302 / 2307
页数：6
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