CONFORMATION OF 3'-NITRO-2',3'-DIDEOXYTHYMIDINE IN CRYSTAL AND SUBSTRATE PROPERTIES OF ITS 5'-TRIPHOSPHATE TOWARD REVERSE TRANSCRIPTASES

被引：0
|
作者：
KUZNETSOVA, EV [1 ]
KUKHANOVA, MK [1 ]
GURSKAYA, GV [1 ]
FEDOROV, II [1 ]
YASKO, MV [1 ]
CHATTOPADHYAYA, D [1 ]
KRAYEVSKY, AA [1 ]
机构：
[1] UPPSALA UNIV,S-75123 UPPSALA,SWEDEN
来源：
MOLECULAR BIOLOGY | 1995年 / 29卷 / 02期
关键词：
DNA POLYMERASE; REVERSE TRANSCRIPTASE; 3'NITRO-2'; 3'-DIDEOXYTHYMIDINE; 5'-TRIPHOSPHATE; TERMINATING SUBSTRATE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It was shown that the conformation of 3'-nitro-2',3'-dideoxythymidine in crystal is close to that of one of the 3'-azido-2',3'-dideoxythymidine forms, This compound terminated DNA synthesis catalyzed by HN reverse transcriptase, and the kinetic parameters of its incorporation into the DNA chain were close to those for 3'-azido-2',3'-dideoxythymidine 5'-triphosphate, 3'-Nitro-2',3'-dideoxythymidine 5'-triphosphate was less active toward avian myeloblastosis virus reverse transcriptase, and did not terminate DNA synthesis catalyzed by DNA polymerases or from human placenta and beta from rat liver, as well as herpes simplex virus type 1 and human cytomegalovirus DNA polymerases and calf thymus terminal deoxynucleotidyl transferase.
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页码：242 / 246
页数：5
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