An indirect ELISA for the diagnosis of brucellosis in sheep

被引:0
|
作者
Alonso, O
Rojas, X
Guzman, E
机构
关键词
Brucella ovis; ELISA; diagnosis;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
A hot saline antigen from Brucella ovis REO 198 was extracted to be used in an indirect ELISA test (ELISA-I) for the diagnosis of sheep brucellosis. This antigen was comparatively used in the ELISA-I and Gel Immune Diffusion test (IDG) in 1181 serum samples belonging to sheep of different epidemiological statuses. The extracted antigen was highly hydrophilic performing the tests easily. The better relative sensitivity showed by the ELISA-I over IDG is discussed.
引用
收藏
页码:113 / 117
页数:5
相关论文
共 50 条
  • [1] EVALUATION OF AN INDIRECT ELISA FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS
    UZAL, FA
    CARRASCO, AE
    ECHAIDE, S
    NIELSEN, K
    ROBLES, CA
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1995, 7 (04) : 473 - 475
  • [2] Assessment of an indirect elisa in milk for the diagnosis of ovine brucellosis
    Biancifiori, F
    Nannini, D
    DiMatteo, A
    Belfiore, P
    [J]. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 1996, 19 (01) : 17 - 24
  • [3] Indirect ELISA for the diagnosis of brucellosis in water buffaloes (Bubalus bubalis) in Italy
    Guarino, A
    Fusco, G
    Di Matteo, A
    Urbani, G
    Condoleo, R
    Serpe, L
    Tittarelli, M
    Di Ventura, M
    Gallo, P
    [J]. VETERINARY RECORD, 2001, 149 (03) : 88 - 90
  • [4] Evaluation of an indirect ELISA kit for the diagnosis of bovine brucellosis in Latin America
    Uzal, FA
    Abalos, P
    Padilla, F
    Rojas, X
    Dajer, A
    Silva, M
    Nielsen, KH
    Wright, PF
    [J]. ARCHIVOS DE MEDICINA VETERINARIA, 1995, 27 : 59 - 63
  • [5] DIAGNOSIS OF HUMAN BRUCELLOSIS WITH ELISA
    GRANFORS, K
    TOIVANEN, A
    [J]. LANCET, 1982, 2 (8299): : 668 - 669
  • [6] Evaluation of an ELISA for the diagnosis of brucellosis
    Mathai, E
    Singhal, A
    Verghese, S
    DLima, D
    Mathai, D
    Ganesh, A
    Thomas, K
    Moses, P
    [J]. INDIAN JOURNAL OF MEDICAL RESEARCH, 1996, 103 : 323 - 324
  • [7] DIAGNOSIS OF HUMAN BRUCELLOSIS WITH ELISA
    SIPPEL, JE
    ELMASRY, NA
    FARID, Z
    [J]. LANCET, 1982, 2 (8288): : 19 - 21
  • [8] Comparison of indirect ELISA in brucellosis diagnosis in vaccinated and non-vaccinated bovine herd
    Jardim, Gustavo Coelho
    Pires, Pedro Paulo
    Mathias, Luis Antonio
    Kuchembuck, Marcio Rubens Graf
    [J]. AGRARIAN, 2009, 2 (05): : 131 - 142
  • [9] Development and Comparative Evaluation of a Competitive ELISA with Rose Bengal Test and a Commercial Indirect ELISA for Serological Diagnosis of Brucellosis
    Tadepalli Mythili
    Lingala Rajendra
    Trangadia Bhavesh
    Dorairajan Thiagarajan
    Villuppanoor Alwar Srinivasan
    [J]. Indian Journal of Microbiology, 2011, 51 : 528 - 530
  • [10] Development and Comparative Evaluation of a Competitive ELISA with Rose Bengal Test and a Commercial Indirect ELISA for Serological Diagnosis of Brucellosis
    Mythili, Tadepalli
    Rajendra, Lingala
    Bhavesh, Trangadia
    Thiagarajan, Dorairajan
    Srinivasan, Villuppanoor Alwar
    [J]. INDIAN JOURNAL OF MICROBIOLOGY, 2011, 51 (04) : 528 - 530