CLONING OF A CDNA-ENCODING A HUMAN DNA-BINDING PROTEIN SIMILAR TO RIBOSOMAL-PROTEIN S1

被引:15
|
作者
EKLUND, EA [1 ]
LEE, SW [1 ]
SKALNIK, DG [1 ]
机构
[1] INDIANA UNIV, SCH MED, HERMAN B WELLS CTR PEDIAT RES, DEPT PEDIAT, INDIANAPOLIS, IN 46202 USA
关键词
GLUTATHIONE S-TRANSFERASE FUSION PROTEIN; LIGAND SCREEN; ESCHERICHIA COLI; SOUTHWESTERN BLOT;
D O I
10.1016/0378-1119(94)00898-3
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We report the cloning of a human complementary DNA that encodes a protein which exhibits 36% identity and 62% similarity to Escherichia coli ribosomal protein S1 (rpS1), including conservation of four copies of an RNA-binding domain, This clone was obtained by ligand-screening a lambda gt11 expression library with a DNA probe derived from the CYBB gene promoter. Electrophoretic mobility shift and Southwestern blot assays confirm DNA binding activity of the protein, which exhibits preferential binding to single-stranded and double-stranded DNA and a low binding affinity for RNA, Hence, the rpS1 protein domain previously identified as an RNA-binding motif can also serve as a DNA-binding domain.
引用
收藏
页码:231 / 235
页数:5
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