A QUICK, SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY FOR MONOETHYLGLYCINEXYLIDIDE AND LIGNOCAINE IN SERUM PLASMA USING SOLID-PHASE EXTRACTION

Recent advances in the investigation of liver disease and transplantation have seen the introduction of lignocaine as a probe of liver function. For this purpose, an assay that is sensitive and rapid is required for the major metabolite of lignocaine, monoethylglycinexylidide (MEGX). We have developed an accurate, low-cost high-performance liquid chromatography (HPLC) method using Bond-Elut phenyl (1 cc) cartridges for sample preparation. The total preparation time for five samples is <10 min and the run time is approximately 10 min/sample. Each cartridge can be used at least four times. Simultaneous measurement of another metabolite of lignocaine, glycinexylidide (GX), can be achieved by adjustment of the mobile phase and flow rate. The chromatogram is monitored with an UV detector at 210 nm. The inter- and intra-assay coefficients of variation for MEGX (10-250-mu-g/L) and lignocaine (100-2,000-mu-g/L) are <9.5 and <2%, respectively, with recoveries for MEGX, trimethoprim (internal standard), and lignocaine all >85%. This method offers a rapid, sensitive assay that is clinically useful in the new role for lignocaine/ MEGX in dynamic liver function testing.