Immobilization of pectinase from Bacillus licheniformis KIBGE-1B21 on chitosan beads for continuous degradation of pectin polymers

被引：25

作者：

Rehman, Haneef Ur ^{[1
]}

Nawaz, Mohammad Asif ^{[2
]}

Aman, Afsheen ^{[2
]}

Baloch, Abdul Hameed ^{[1
]}

Qader, Shah Ali Ul ^{[2
]}

机构：

[1] Lasbela Univ Agr, Fac Agr, Water & Marine Sci LUAWMS, Balochistan 90150, Pakistan

[2] Univ Karachi, Karachi Inst Biotechnol & Genet Engn KIBGE, Karachi 75270, Pakistan

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2014年 / 3卷 / 04期

关键词：

Immobilization; Chitosan beads; Pectinase; Degradation; Pectin polymers;

D O I：

10.1016/j.bcab.2014.09.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pectinase catalyzes the degradation of pectin substances and has been used in various food and textile industries. In this study, pectinase from Bacillus lichenifonnis KIBGE-1B21 was immobilized on chitosan beads using formaldehyde as a crosslinking agent. Various concentration of formaldehyde was tried during immobilization procedure and 10% was found to immobilize maximum pectinase. The optimum crosslinking time and temperature for the activation of chitosan beads were observed to be 3.0 h and 30 degrees C, respectively. The immobilization did not influence the optimum pH and temperature of pectinase for maximum activity and both the native and immobilized pectinase showed maximum activity at same pH and temperature. However, the stability of pectinase against different pH and temperatures was increased after immobilization. The chitosan immobilized pectinase showed excellent recycling efficiency and retained more than 90% of its initial activity during 4th cycle. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：282 / 287

页数：6

共 6 条

[1] Degradation of complex carbohydrate: Immobilization of pectinase from Bacillus licheniformis KIBGE-IB21 using calcium alginate as a support
Rehman, Haneef Ur
Arnan, Afsheen
Silipo, Alba
Ul Qader, Shah Ali
Molinaro, Antonio
Ansari, Asma
[J]. FOOD CHEMISTRY, 2013, 139 (1-4) : 1081 - 1086
[2] Polygalacturonase: Production of pectin depolymerising enzyme from Bacillus licheniformis KIBGE IB-21
Rehman, Haneef Ur
Ul Qader, Shah Ali
Aman, Afsheen
[J]. CARBOHYDRATE POLYMERS, 2012, 90 (01) : 387 - 391
[3] Immobilization of pectin degrading enzyme from Bacillus lichernformis KIBGE IB-21 using agar-agar as a support
Rehman, Haneef Ur
Aman, Afsheen
Zohra, Raheela Rahmat
Ul Qader, Shah Ali
[J]. CARBOHYDRATE POLYMERS, 2014, 102 : 622 - 626
[4] Immobilization of Endo (1→4) β-D-Glucanase from Bacillus Licheniformis KIBGE-IB2 Using Agar-Agar as Support for Continuous Use
Karim, Asad
Ul Qader, Shah Ali
Nazwaz, Asif
Aman, Afsheen
[J]. IBIC2014: 4TH INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY, 2014, 38 : 409 - 414
[5] Association of Laccase from Bacillus cereus O2-B and Pseudomonas aeruginosa O1-P with the bio-degradation of polymers: an in vitro to in silico approach
Farveen, M. Shafana
Madhavan, Thirumurthy
Narayanan, Rajnish
[J]. BIODEGRADATION, 2023, 34 (04) : 383 - 403
[6] Association of Laccase from Bacillus cereus O2-B and Pseudomonas aeruginosa O1-P with the bio-degradation of polymers: an in vitro to in silico approach (vol 34, pg 383, 2023)
Shafana Farveen, Mohamed
Madhavan, Thirumurthy
Narayanan, Rajnish
[J]. BIODEGRADATION, 2024,

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