MODULATION BY PROGESTERONE OF INTERLEUKIN-6 PRODUCTION BY GINGIVAL FIBROBLASTS

被引:65
|
作者
LAPP, CA [1 ]
THOMAS, ME [1 ]
LEWIS, JB [1 ]
机构
[1] MED COLL GEORGIA, DEPT PERIODONTOL, AUGUSTA, GA 30912 USA
关键词
PROGESTERONE; ADVERSE EFFECTS; ESTROGEN; INTERLEUKIN-6; GINGIVITIS; ETIOLOGY; FIBROBLASTS; PREGNANCY; INFLAMMATION;
D O I
10.1902/jop.1995.66.4.279
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
THE GINGIVITIS ASSOCIATED WITH PREGNANCY has been attributed to increased concentrations of circulating estrogen and/or progesterone. However, the mechanism by which these steroids increase gingival inflammation is not known. Interleukin-6 (IL-6), a pleiotropic cytokine produced by many cell types including human gingival fibroblasts (hGF), is secreted in response to inflammatory challenges such as bacterial lipopolysaccharide and interleukin-l (IL-1). This study tested the hypothesis that progesterone could modulate the local production of IL-6 by hGF. The effects of progesterone on IL-6 production were measured in vitro in serum-free, phenol red-free medium to eliminate possible effects of such medium additives. The concentration of IL-6 secreted into supernatant medium after a 24 hour challenge with IL-1 beta was estimated by radioimmunoassay. Total RNA from steroid-treated hGF was probed for IL-6 mRNA. In serum-free medium, progesterone dose-dependently and significantly (P<0.05) inhibited IL-6 production by hGF, as did the glucocorticoids hydrocortisone (HC) and dexamethasone. At progesterone concentrations common in late pregnancy, IL-6 production was reduced to levels 40 to 50% of control. In addition, mRNA was significantly down-regulated by progesterone and HC, at both basal levels and after IL-1 beta challenge. These results suggest that high levels of progesterone during pregnancy affect the development of localized inflammation by: down-regulation of IL-6 production, rendering the gingiva less efficient at resisting the inflammatory challenges produced by bacteria.
引用
收藏
页码:279 / 284
页数:6
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