A STUDY ON TRANSMEMBRANE ORGANIZATION OF THE C-TERMINAL DOMAIN OF THE NA+,K+-ATPASE WITH POLYCLONAL ANTIBODIES TO THE CLOSE-TO-THE-MEMBRANE FRAGMENTS EXPRESSED IN ESCHERICHIA-COLI

被引：0

作者：

LARIN, DI ^{[1
]}

SHAKHAPARONOV, MI ^{[1
]}

KOSTINA, MB ^{[1
]}

MODYANOV, NN ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,MM SHEMYAKIN & YA OVCHINNIKOV INST BIOORGAN CHEM,16-10 MIKLUKHO MAKLAYA UL,V437,MOSCOW 117871,RUSSIA

来源：

BIOORGANICHESKAYA KHIMIYA | 1994年 / 20卷 / 8-9期

关键词：

NA+; K+-ATPASE; ANTIBODIES; FUSION PROTEINS;

D O I：

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中图分类号：

学科分类号：

摘要：

The fragments 825-842, 868-886, 928-945, 946-967, 962-978 of the alpha-subunit of the porcine Na+,K+-ATPase were expressed in E. coli as fusion proteins with human tumour necrosis factor. Polyclonal antibodies were obtained to these fragments. The location of the chosen fragments according to the plasma membrane was determined with the polyclonal antibodies by ELISA on intact, lysed or solubilized pig kidney embryo cells. The fragments 825-842, 928-945, and 962-978 contained intracellular epitopes and did not contain extracellular ones; the fragments 868-886 and 946-967 did not contain any exposed epitopes.

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页码：833 / 841

页数：9

共 17 条

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