A COLORIMETRIC METHOD FOR THE DETERMINATION OF LIPOXYGENASE ACTIVITY SUITABLE FOR USE IN A HIGH-THROUGHPUT ASSAY FORMAT

被引：59
|
作者：
WASLIDGE, NB [1 ]
HAYES, DJ [1 ]
机构：
[1] WELLCOME RES LABS, ENZYMOL GRP, BECKENHAM BR3 3BS, KENT, ENGLAND
来源：
ANALYTICAL BIOCHEMISTRY | 1995年 / 231卷 / 02期
关键词：
D O I：
10.1006/abio.1995.0063
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and sensitive method for measuring the activity of lipoxygenase is described. The assay is based on the principle that under acidic conditions a lipid hydroperoxide can oxidize Fe2+ to Fe3+ which then oxidizes xylenol orange to form a product that absorbs strongly in the visible region (see Z-Y. Jiang, A. C. S. Woollard, and S. P. Wolff, Lipids 26, 853-856, 1991). This methodology was modified to measure lipoxygenase activity and the system was optimized using platelet 12-lipoxygenase. This assay is suitable for use in a 96-well microtiter plate and may be utilized as a high throughput screen for the identification of novel lipoxygenase inhibitors. (C) 1995 Academic Press, Inc.
引用
收藏
页码：354 / 358
页数：5
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