OCCURRENCE OF 28-CARBON AND 27-CARBON ECDYSTEROIDS AND STEROLS IN DEVELOPING WORKER PUPAE OF THE LEAF-CUTTING ANT ACROMYRMEX-OCTOSPINOSUS (REICH) (HYMENOPTERA, FORMICIDAE, ATTINI)

被引：12
|
作者：
MAURER, P
ROYER, C
MAUCHAMP, B
PORCHERON, P
DEBIEU, D
RIBA, G
机构：
[1] INRA,PHYTOPHARM LAB,F-78000 VERSAILLES,FRANCE
[2] INST NATL AGRON PARIS GRIGNON,ZOOL LAB,PARIS,FRANCE
[3] UNIV PARIS 06,EVOLUT LAB,CYTOPHYSIOL ARTHROPODES LAB,F-75230 PARIS 05,FRANCE
[4] INRA,PHYSIOL INSECTE LAB,F-78000 VERSAILLES,FRANCE
来源：
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 1991年 / 16卷 / 01期
关键词：
ECDYSTEROIDS; MAKISTERONE-A; 20-HYDROXYECDYSONE; ENZYME IMMUNOASSAY (EIA);
D O I：
10.1002/arch.940160102
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The major ecdysteroids in large worker pupae of the leaf-cutting ant Acromyrmex octospinosus were characterized at the peak ecdysteroid concentration by using high-performance liquid chromatography, enzyme immunoassay, and mass spectrometry. In decreasing amounts, they were determined to be makisterone A, an unidentified C-28 ecdysteroid bearing a molecular weight of 494, 20-hydroxyecdysone (ratio of 1 to 6 as compared to makisterone A), and putative but negligible ecdysone. The presence of both C-28 and C-27 ecdysteroids is discussed in relation to the content of 4-desmethylsterols determined by gas chromatography and mass spectrometry to be ergosta-5,7,24 (28)-trien-3-beta-ol, ergosterol, ergosta-5,7-dien-3-beta-ol and ergosta-7,24(28)-dien-3-beta-ol for the main sterols, and with a small amount of cholesterol.
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页码：1 / 9
页数：9
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