SOLID-PHASE EXTRACTION OF ASCORBIC-ACID 2-SULFATE FROM CYSTS OF THE BRINE SHRIMP ARTEMIA-FRANCISCANA

Two procedures are described for the solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia, The first one is based on reversed-phase ion pair extraction on an octadecylsilica cartridge using tetrabutylammonium as the counterion, followed by elution with methanol. In the second procedure ascorbic acid 2-sulfate is retained on a DEAE silica cartridge and eluted with 0.1 M sodium salicylate. Both approaches were used as part of a quantitative reversed-phase ion pair liquid chromatographic method with detection at 254 nm. The anion exchange extraction procedure is superior to its ion pair counterpart in terms of recovery (88.7 +/- 3.5% versus 63.0 +/- 12.7%), reproducibility (CVs of 3.6-7.7% versus 6.3-18.7%), and simplicity.