PURIFICATION AND CHARACTERIZATION OF A LECTIN FROM MACROBRACHIUM-ROSENBERGII (CRUSTACEA, DECAPODA) HEMOLYMPH

被引：47
|
作者：
VAZQUEZ, L
MASSO, F
ROSAS, P
MONTANO, LF
ZENTENO, E
机构：
[1] Univ Nacl Autonoma Mexico, FAC MED, DEPT BIOQUIM, MEXICO CITY, MEXICO
[2] UNIV AUTONOMA ESTADO MORELOS, BIOL EXPTL LAB, CUERNAVACA, MORELOS, MEXICO
[3] INST NACL ENFERMEDADES RESP, DEPT BIOQUIM, TLALPAN, MEXICO
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 105卷 / 3-4期
关键词：
D O I：
10.1016/0305-0491(93)90097-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. We have purified a 19 kDa lectin from the freshwater prawn Macrobrachium rosenbergii by single-step affinity chromatography on a rat stroma column. 2. The lectin is a glycoprotein composed of two monomeric subunits of 9.6 kDa bound by a disulfide bridge; 7% of its contents are carbohydrates. 3. It has an S20,w value of 1.4, a pI of 5.4-6.1, and is rich in glycine, serine, glutamic and aspartic acid residues. 4. It requires divalent cations to function. 5. It recognizes sialic acid. 6. The hemagglutinating and hapten inhibition assays strongly suggest that it is 9-O-acetylsialic acid residue-specific.
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页码：617 / 623
页数：7
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