SYNAPSE FORMATION IN DISSOCIATED CELL-CULTURES OF EMBRYONIC CHICK CEREBRAL NEURONS

被引:25
|
作者
TOKIOKA, R [1 ]
MATSUO, A [1 ]
KIYOSUE, K [1 ]
KASAI, M [1 ]
TAGUCHI, T [1 ]
机构
[1] OSAKA UNIV,FAC ENGN,DEPT BIOPHYS ENGN,TOYONAKA,OSAKA 560,JAPAN
来源
DEVELOPMENTAL BRAIN RESEARCH | 1993年 / 74卷 / 01期
关键词
WHOLE-CELL PATCH CLAMP; SYNAPTOGENESIS; CHICK EMBRYONIC NEURON; GAMMA-AMINOBUTYRIC ACID; GLUTAMATE; CELL CULTURE;
D O I
10.1016/0165-3806(93)90096-S
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The development of synapses was confirmed in the primary cultures of dissociated cerebral cortex neurons from chick embryos. Whole-cell patch clamp recording applied to dissociated neurons from 6- to 12-day-old embryos revealed that these neurons form functional synapses. In these cultures, both excitatory and inhibitory postsynaptic responses were observed. Synaptogenesis in our cultures seemed to be in proportion to the embryonic equivalent days, which are the sum of the days in incubation and culture.
引用
收藏
页码:146 / 150
页数:5
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