THE STUDY OF ENTEROTOXIGENICITY OF THE BIOTYPE 1A YERSINIA ENTEROCOLITICA

The representatives of Y. enterocolitica biotype 1A which are considered as nonpathogenic microorganisms were tested for production of the thermostable enterotoxin YST B (Yersinia Stable Toxin). This toxin is characterized by strong toxic action and it can bring on diarrhea in human and animals. The chromosome gene of thermostable enterotoxin ystB was detected by PCR in 87.1% out of 116 studied strains of different origin and territorial isolation. To determine toxin production in vitro the studied strains cultivated in various conditions: in 26 degrees C and 37 degrees C in usual culture medium and in 37 degrees C in the medium corresponded to the content of intestine. In part of the studied strains the toxin production was revealed on the model of newborn mice in both temperature regimes of cultivation 26 degrees C and 37 degrees C. The study of toxin production in representatives of Y. enterocolitica biotype 1A showed their possible role as etiological agents of diarrhea.