PURIFICATION AND CHARACTERIZATION OF INVERTASE FROM ASPERGILLUS-NIGER

被引：44
|
作者：
RUBIO, MC
MALDONADO, MC
机构：
[1] Department of Industrial Microbiology, Faculty of Biochemistry and Pharmacy, National University of Tucumán, Tucumán, 4000
来源：
CURRENT MICROBIOLOGY | 1995年 / 31卷 / 02期
关键词：
D O I：
10.1007/BF00294280
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Invertase produced by a strain of Aspergillus niger showed the following main characteristics: maximum activity at 60 degrees C, pH 5.0; K-m with sucrose as substrate, 0.0625 mM; V-max 0.013 mol/min; and free energy 9132 cal/mol. The metal ions and p-chloromercuribenzoate (PCMB) acted as inhibitors respectively.
引用
收藏
页码：80 / 83
页数：4
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