PLATELET-DERIVED GROWTH-FACTOR GENE-EXPRESSION IN CULTURED HUMAN RETINAL-PIGMENT EPITHELIAL-CELLS

被引：47
|
作者：
YOSHIDA, M
TANIHARA, H
YOSHIMURA, N
机构：
[1] Department of Ophthalmology, Kyoto University Faculty of Medicine, Kyoto
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 01期
关键词：
D O I：
10.1016/0006-291X(92)91526-V
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gene expression of platelet-derived growth factor (PDGF) and its receptors in cultured human retinal pigment epithelial (RPE) cells was studied by using semiquantitative polymerase chain reaction. The RPE cells were found to express PDGF A- and B-chain genes as well as α- and β-receptor genes with dominant expression of B-chain and β-receptor isoforms. Phorbol myristate acetate (PMA) and thrombin increased the expression of PDGF β-chain gene to 19.8 ± 1.75 and 15.9 ± 1.84 fold (n=3) of the control without affecting β-receptor gene expression. PDGF produced by the RPE cells may play an important role in the pathogenesis of some ocular proliferative diseases. © 1992.
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收藏
页码：66 / 71
页数：6
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