SEPARATION OF METHANOTROPHIC BACTERIA BY USING PERCOLL AND ITS APPLICATION TO ISOLATION OF MIXED AND PURE CULTURES

被引：16
|
作者：
PUTZER, KP
BUCHHOLZ, LA
LIDSTROM, ME
REMSEN, CC
机构：
[1] UNIV WISCONSIN,CTR GREAT LAKES STUDIES,MILWAUKEE,WI 53201
[2] UNIV WISCONSIN,DEPT BIOL SCI,MILWAUKEE,WI 53201
[3] CALTECH,KECK LABS,PASADENA,CA 91125
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 12期
关键词：
D O I：
10.1128/AEM.57.12.3656-3659.1991
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.
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页码：3656 / 3659
页数：4
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