Production and partial characterization of extracellular glucose isomerase using thermophilic Bacillus sp. isolated from agriculture land

被引：4

作者：

Sukumar, MohanaSundaram ^{[1
]}

Jeyaseelan, Arun ^{[1
]}

Sivasankaran, Thirumalai ^{[1
]}

Mohanraj, Pennarasi ^{[1
]}

Mani, Prasanna ^{[1
]}

Sudhakar, Gowthamraj ^{[1
]}

Arumugam, Vimalraj ^{[1
]}

Bakthavachalu, Sasirekha ^{[1
]}

Ganeshan, Alagulakshmi ^{[1
]}

Susee, Monika ^{[1
]}

机构：

[1] Karpaga Vinayaga Coll Engn & Technol, Dept Biotechnol, Maduranthagam 603308, Tamil Nadu, India

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2013年 / 2卷 / 01期

关键词：

Extracellular glucose isomerase; Thermostable; Bacillus sp; Fermentation; Stability;

D O I：

10.1016/j.bcab.2012.10.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this investigation, glucose isomerase was extracted from Bacillus sp., isolated from agricultural soil sample and purified further by ammonium sulfate fractionation followed by gel filtration chromatography using Sephadex G-25. The purified enzyme over crude was further subjected to partial characterization which includes the assessment of influence of pH, temperature, incubation time and metal ions on the stability and activity of glucose isomerase was observed. As a result, it was found that, glucose isomerase isolated from the thermophilic Bacillus sp. enumerated from the agricultural soil showed maximal stability at pH 8.0 and at temperature 70 C within 35 min. It was also found that, at the minimum concentration of 5 mM Mg2+ Co2+ and Mn2+ showed 41%-59% of enzyme activity. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：45 / 49

页数：5

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