SIMULTANEOUS DETERMINATION OF TRALOMETHRIN, DELTAMETHRIN, AND RELATED-COMPOUNDS BY HPLC WITH RADIOMETRIC DETECTION

Analytical methodologies for the simultaneous determination of the synthetic pyrethroids tralomethrin, deltamethrin, and decamethrinic acid (Br2CA) in water, sediment, and fish tissue by HPLC with radiometric detection (HPLC-RAM) are described. The separation of the three compounds was achieved using a hexane/dioxane gradient solvent system on a silica column. This solvent system could also be used to resolve alpha-(R)-deltamethrin from trans-deltamethrin. Water, sediment, and fish tissue sample preparation involved relatively simple solvent extractions. The quantitation of these pyrethroids was validated at low nanograms per liter (parts per trillion) concentrations for water samples and low nanograms per gram (parts per billion) levels for sediment samples. The objective of this work was to provide a relatively simple, rapid, and sensitive method which permits the simultaneous identification and quantification of radiolabeled tralomethrin, deltamethrin, and their related compounds.