STEREOSELECTIVE SYNTHESIS OF STABLE ISOTOPE-LABELED L-ALPHA-AMINO ACIDS - BIOSYNTHESIS OF H-2-LABELED, C-13-LABELED, AND N-15-LABELED L-SERINES

被引：9

作者：

HANNERS, J ^{[1
]}

GIBSON, R ^{[1
]}

VELARDE, K ^{[1
]}

HAMMER, J ^{[1
]}

ALVAREZ, M ^{[1
]}

GRIEGO, J ^{[1
]}

UNKEFER, CJ ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,NATL STABLE ISOTOPES RESOURCE,INC-4 GRP,MS C345,LOS ALAMOS,NM 87545

来源：

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 1991年 / 29卷 / 07期

关键词：

L-[1-C-13]SERINE; L-[2-C-13]SERINE; L-[3-C-13]SERINE; L-[2,3-C-13(2)]SERINE; L-[3-H-2(2); C-13]SERINE;

D O I：

10.1002/jlcr.2580290708

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a stereospecific biosynthesis of C-13 and N-15-labeled L-serine which involves the serine-type methylotroph Methylobacterium extorquens AM1. In this biosynthesis, C-3 of serine is derived from methanol while C-2, C-1 and the a-amino group are derived from glycine. By starting with the appropriate labeling precursor, we can produce any of the H-2, C-13 and/or N-15 isotopomers of L-serine. Using a 5-L culture, 40-50 millimoles of L-serine are produced; L-serine and glycine are recovered from the growth medium and then separated chromatographically.

引用

页码：781 / 790

页数：10

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