Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro

被引：7
|
作者：
Puri, Gopal [1 ]
Chaudhary, S. S. [1 ]
Singh, V. K. [1 ]
Sharma, A. K. [1 ]
机构：
[1] Navsari Agr Univ, Vanbandhu Coll Vet Sci & Anim Husb, Dept Vet Physiol & Biochem, Navsari 396450, Gujarat, India
来源：
VETERINARY WORLD | 2015年 / 8卷 / 02期
关键词：
buffalo oocytes; estrus buffalo serum; fetal bovine serum; in vitro maturation;
D O I：
10.14202/vetworld.2015.143-146
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Aim: The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. Materials and Methods: Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitro culture in CO 2 incubator. Results: The average percentage of in vitro matured oocytes in FBS was 83.80%, and EBS was 77.45%, respectively. The results revealed a significant (p<0.05) increase in maturation rate of oocytes in FBS than EBS. Conclusion: Buffalo oocytes were better in vitro matured in FBS than EBS.
引用
收藏
页码：143 / 146
页数：4
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