Analysis of the related substances in phosphorothioate antisense oligonucleotide FT19

被引：2

作者：

Zhang Hongyan ^{[1
]}

Lu Dandan ^{[1
]}

Wu Lixia ^{[1
]}

Zhou Zhe ^{[1
]}

Wang Shengqi ^{[1
]}

机构：

[1] Beijing Inst Radiat Med, Beijing 100850, Peoples R China

来源：

CHINESE JOURNAL OF CHROMATOGRAPHY | 2008年 / 26卷 / 05期

关键词：

anion exchange high performance liquid chromatography (AX-HPLC); capillary gel electrophoresis (CGE); phosphorothioate oligonucleotide; related substances;

D O I：

10.1016/S1872-2059(08)60028-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method using anion exchange high performance liquid chromatography (AXHPLC) and capillary gel electrophoresis (CGE) was established for the analysis of the related substances in phosphorothioate antisense oligonucleotide FT19 for quality control. The FT19 and its analogs of partial phosphodiester compounds (P=0), as well as deletion sequences (n - 1) were analyzed. AX-HPLC with the DNA Pac PA-100 column (4 mm x 250 mm) was used for the separation of phosphorothioate modifications. The size of the capillary column used in the CGE was 31 cm x 100 mu m with an effective length of 20 cm. It was found that full phosphorothioate and partial phosphodiester oligonucleotides of the same length were successfully separated, and the deletion sequences (n - 1) and the full-length sequence n were successfully separated in CGE. The results demonstrated that the related substances of phosphorothioate oligonucleotides can be well analyzed by AX-HPLC and CGE.

引用

页码：540 / 543

页数：4

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