NO LITHOLYTIC EFFECT OF ZNSO4 AND CUSO4 ON EXPERIMENTAL LITHIASIS IN RATS

Seventy two male Wistar-strain rats were fed lithogenic diet with ethylene-glycol within three weeks. At the end of this treatment, six rats were killed in order to determine the oxalate and calcium concentrations in renal tissue. Remained rats was randomly divided in four series, each serie consisting of three groups. In first serie (T), the animals were treated with distilled water; in the second (D1Zn), the animals were treated intramuscularly with the zinc at the rate of 24 mu g per 100 grams of body weight and per day, in the third (D2Zn), 240 mu g of zinc were administrated to animals and in the last serie (D2Cu), the animals were treated at the same dose as the previous serie, but with the copper. The groups wich making up each serie were killed successively at the 5 th, 10 th and 15 th day after ending treatment with ethyleneglycol in order to determine urinary pH, percentage of water in renal tissue, uremia and concentrations of oxalate and calcium in renal tissue. Then, the comparisons of means were carried out, at each time, between different treated groups and reference group wich was treated with distilled water. Litholytic effect was found in all series, including that which had been treated with distilled water. However, compared to reference animals, no acceleration of litholytic process was induced by zinc or copper. The high doses used in these experimentations proved therefore that calcium oxalate calculi were insoluble by zinc and copper.