DNA-FINGERPRINTING DETECTS GENETIC-VARIABILITY IN THE PEARL-MILLET DOWNY MILDEW PATHOGEN (SCLEROSPORA-GRAMINICOLA)

被引：28
|
作者：
SASTRY, JG
RAMAKRISHNA, W
SIVARAMAKRISHNAN, S
THAKUR, RP
GUPTA, VS
RANJEKAR, PK
机构：
[1] INT CROPS RES INST SEMI ARID TROP,PATANCHERU 502324,ANDHRA PRADESH,INDIA
[2] NATL CHEM LAB,PLANT MOLEC BIOL UNIT,POONA 411008,MAHARASHTRA,INDIA
来源：
THEORETICAL AND APPLIED GENETICS | 1995年 / 91卷 / 6-7期
关键词：
DOWNY MILDEW; MICROSATELLITES; DNA FINGERPRINTING; PEARL MILLET;
D O I：
10.1007/BF00223892
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Genetic variability in six host genotype-specific pathotypes of pearl millet downy mildew pathogen S. graminicola was studied at the molecular level using mini- and micro-satellites. Our results indicated that microsatellites (GAA)(6), (GACA)(4), and especially (GATA)(4) were quite informative and showed high levels of polymorphism among the pathotypes. The six pathotypes could be classified into five groups based on the cluster analysis of their genetic similarities, thereby confirming the existence of distinct host genotype-specific virulence in S. graminicola pathotypes. We demonstrate, for the first time, the use of DNA fingerprinting to detect genetic variation in downy mildew fungus of pearl millet.
引用
收藏
页码：856 / 861
页数：6
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