PREPARATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO SUBSTANCE-P

被引：0
|
作者：
FRICKEY, PH
YANNI, JM
APPELL, K
机构：
[1] ALCON LABS INC, FT WORTH, TX 76134 USA
[2] STERLING DRUG CO, STERLING RES GRP, MALVERN, PA 19355 USA
来源：
HYBRIDOMA | 1991年 / 10卷 / 06期
关键词：
D O I：
10.1089/hyb.1991.10.685
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A series of mouse hybridomas that produced monoclonal antibodies reactive with Substance P was prepared in an attempt to find a surrogate NK-1 receptor. When the binding profile of one particular antibody, SubP14.36.1, was compared in a rank order plot with NK-1 receptors against various agonists and antagonists of Substance P, a high correlation was found between these two binding structures. In addition, this monoclonal antibody inhibited a functional assay for Substance P. This result indicated that the pharmacological effects of this peptide can be blocked by an antibody (Ab).
引用
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页码：685 / 694
页数：10
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