COMPETITIVE-PROTEIN-BINDING ASSAY FOR ACTIVIN-A/EDF USING FOLLISTATIN DETERMINATION OF ACTIVIN LEVELS IN HUMAN PLASMA

被引：21
|
作者：
DEMURA, R
SUZUKI, T
TAJIMA, S
MITSUHASHI, S
ODAGIRI, E
ETO, Y
SUGINO, H
DEMURA, H
机构：
[1] TOKYO WOMENS MED COLL, INST CLIN ENDOCRINOL, TOKYO 162, JAPAN
[2] AJINOMOTO CO INC, CENT RES LABS, KAWASAKI, KANAGAWA 210, JAPAN
[3] UNIV TOKUSHIMA, INST ENZYME RES, TOKUSHIMA 770, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 185卷 / 03期
关键词：
D O I：
10.1016/0006-291X(92)91746-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive and specific protein binding assay for activin A EDF (activin) was developed using follistatin as a binding protein and [125I] labelled activin as a tracer. As 50% acetonitrile (CH3CN) separated free and follistatin-bound activin, plasma pretreated with an equal volume of CH3CN was used as the assay sample and B F separation was also done with 50% CH3CN. The recovery of the assay was 85.0% and its sensitivity was 0.5 ng/ml. Crossreactivity with inhibin A was 1.8%. The mean plasma level of follistatin-free activin in normal subjects was 1.3±0.7 (M±SD) ng/ml. Plasma free activin levels were generally elevated in patients with chronic renal failure or hematological diseases associated with anemia. © 1992.
引用
收藏
页码：1148 / 1154
页数：7
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