RESOLUTION OF COMPLEX-MIXTURES OF FLAVONOID AGLYCONES BY ANALYSIS OF GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC DATA

Forty-nine flavones, flavonols, flavanones and chalcones were analysed without derivatization by GC and GC-MS using an OV-1 capillary column. Retention times are affected by the number, position and type of the substituents. The mass spectra show the same typical fragmentation pattern as obtained by the direct inlet method. The effectiveness of the proposed method is demonstrated by the GC and GC-MS analysis of a plant extract in which 21 different flavonoid aglycones could be identified.