CHARACTERIZATION OF FUNCTIONAL HUMAN ERYTHROCYTE-TYPE GLUCOSE TRANSPORTER (GLUT1) EXPRESSED IN INSECT CELLS USING A RECOMBINANT BACULOVIRUS

被引：18
|
作者：
YI, CK
CHARALAMBOUS, BM
EMERY, VC
BALDWIN, SA
机构：
[1] UNIV LONDON,ROYAL FREE HOSP,SCH MED,DEPT BIOCHEM,ROWLAND HILL ST,LONDON NW3 2PF,ENGLAND
[2] UNIV LONDON,ROYAL FREE HOSP,SCH MED,DIV COMMUNICABLE DIS,LONDON NW3 2PF,ENGLAND
[3] UNIV LONDON,ROYAL FREE HOSP,SCH MED,DEPT PROTEIN & MOLEC BIOL,LONDON NW3 2PF,ENGLAND
来源：
BIOCHEMICAL JOURNAL | 1992年 / 283卷
基金：
英国惠康基金;
关键词：
D O I：
10.1042/bj2830643
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The human erythrocyte-type glucose transporter (GLUT1) has been abundantly expressed in insect cells by using a recombinant baculovirus. At 4 days after infection with the virus, the insect cell-surface and intracellular membranes were found to contain > 200 pmol of D-glucose-sensitive binding sites for the transport inhibitor cytochalasin B per mg of protein. The characteristics of binding were identical with those of the erythrocyte transporter, although the two proteins differed substantially in apparent M(r), probably as a result of glycosylation differences.
引用
收藏
页码：643 / 646
页数：4
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