THE USEFULNESS OF SEQUENCE-ANALYSIS OF AMPLIFIED DNA FOR THE IDENTIFICATION OF DELTA CHAIN VARIANTS

被引：9
|
作者：
LI, HW
CODRINGTON, JF
SCHILIRO, G
WADSWORTH, LD
BERIS, P
ADEKILE, A
HUISMAN, THJ
机构：
[1] UNIV CATANIA,DIPARTMENTO PEDIAT,CATTEDRA EMATOL PEDIAT,I-95125 CATANIA,ITALY
[2] BRITISH COLUMBIA CHILDRENS HOSP,DIV HEMATOPATHOL IMMUNOHEMATOL,VANCOUVER V6H 3V4,BC,CANADA
[3] UNIV GENEVA,HOP CANTONAL,DIV HEMATOL,CH-1211 GENEVA 4,SWITZERLAND
[4] UNIV GENEVA,HOP CANTONAL,CTR TRANSFUS SANGUINE,CH-1211 GENEVA 4,SWITZERLAND
来源：
HEMOGLOBIN | 1991年 / 15卷 / 1-2期
关键词：
D O I：
10.3109/03630269109072486
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method of identifying delta-chain variants using relatively small volumes of blood is described. The procedure consists of the amplification of two segments of genomic DNA with two sets of delta-chain specific primers and sequencing of the three exons (exons 1, 2, and 3) which are part of the amplified DNA segments. Data for three delta-chain variants present in seven adult heterozygotes are presented.
引用
收藏
页码：77 / 84
页数：8
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