EFFECT OF AGE ON THE EXPRESSED B-CELL REPERTOIRE - ROLE OF B-CELL SUBSETS

被引:45
|
作者
HU, A [1 ]
EHLEITER, D [1 ]
BENYEHUDA, A [1 ]
SCHWAB, R [1 ]
RUSSO, C [1 ]
SZABO, P [1 ]
WEKSLER, ME [1 ]
机构
[1] CORNELL UNIV, MED CTR, COLL MED, DEPT MED, NEW YORK, NY 10021 USA
关键词
CD5+ B-CELLS; FOREIGN ANTIGENS; IMMUNE SENESCENCE; MICE;
D O I
10.1093/intimm/5.9.1035
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Aged humans and experimental animals are impaired in their responses to most foreign antigens although they produce greater amounts of autoantibodies. We have examined the effect of age on the production of antibodies to a prototypic foreign antigen, sheep erythrocytes (SRBC), and to a prototypic autoantigen, bromelain-treated mouse erythrocytes (BrMRBC), in young and old mice before and after immunization with SRBC. Old mice express more anti-BrMRBC plaque-forming cell (PFC) antibodies before and an even greater number after immunization with SRBC than young mice. Conversely, old mice produce far fewer anti-SRBC PFC than young mice following immunization with SRBC. We hypothesized that the differences in the responses of old mice to BrMRBC and SRBC reflects differences in the activity of CD5+ and CD5- B cells. To test this hypothesis we immunized young and old mice with foreign antigens reported (and confirmed in our studies) to stimulate CD5+ B cells [TNP - ficoll and phosphorylcholine - keyhole limpet hemocyanin (KLH)] or CD5- B cells (SRBC and TNP - KLH). We found that the PFC response of old mice to antigens mediated by CD5+ B cells was equal to or greater than that of young mice. In contrast the PFC response of old mice induced by antigens mediated by CD5- B cells was only 10% that of young mice. Thus it appears that the immune response of old mice is well maintained for antigens which elicit a CD5+ B cell response but not for those which elicit a CD5- B cell response. This conclusion is supported by our finding that a higher percentage of splenic B cells from old compared to young mice express that V(H)11 Igh gene family. This VH gene family is preferentially expressed by CD5+ B cells.
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页码:1035 / 1039
页数:5
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