DETECTION OF IMMUNOREACTIVE SCHIZOPHYLLAN BY SOLID-PHASE ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：0
|
作者：
HIRATA, A
ITOH, W
KOMODA, M
TABATA, K
ITOYAMA, S
SUGAWARA, I
机构：
[1] SAITAMA MED SCH, SAITAMA MED CTR,DEPT PATHOL,1981 KAMODA, TSUJIDO CHO, KAWAGOE, SAITAMA 350, JAPAN
[2] TAITO CO LTD, RES LAB, NAGATA KU, KOBE 653, JAPAN
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1993年 / 16卷 / 11期
关键词：
SCHIZOPHYLLAN; MONOCLONAL ANTIBODY; ENZYME-LINKED IMMUNOSORBENT ASSAY;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In order to detect immunoreactive schizophyllan (SPG), one of the beta-1,3-glucans in solution, a murine anti-schizophyllan monoclonal antibody, SPG1-HS, was used as a primary antibody for solid-phase enzyme-linked immunosorbent assay (ELISA). Polysaccharide-bovine serum albumin (BSA) and polysaccharide-bovine hemoglobin (Hb) conjugate were prepared as an antigen. The absorbance at 490 nm in ELISA was directly proportional to the SPG concentration in SPG-BSA or SPG-Hb conjugates, allowing the accurate measurement of SPG at concentrations of more than 1.0 mug/ml. Also, this ELISA detected SPG in SPG-BSA and SPG-Hb conjugates in human serum in a similar manner. These results suggest that this ELISA is applicable for the determination of SPG concentration in SPG-BSA and SPG-Hb conjugates in human tissue and blood.
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页码：1091 / 1093
页数：3
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