EXAMINATION OF NEW STATIONARY PHASES FOR THE REVERSED-PHASE CHROMATOGRAPHY OF PEPTIDES

Some new HPLC-phases (Polyencap, Hypercarb, Poly F, PRP-infinity) have been assessed for their suitability in the separation of peptides. Test criteria were peptide recovery, separation-efficiency, selectivity and stability. The eluents TFA/acetonitrile and acetate-buffer/propanol are compared. Of interest are the stability and recovery of Polyencap, the high separation efficiency of the standard RP-8 silica-based phase (Nucleosil C8) and the unusual peak-width of PRP-infinity. Acceptable conditions for peptide separations may be provided by Polyencap, but stability still has to be improved.