AT(D)PMG-INDUCED DISSOCIATION OF THE ALPHA-3-BETA-3 COMPLEX OF THE F1-ATPASE FROM A THERMOPHILIC BACILLUS PS3 INTO ALPHA-1-BETA-1 HETERODIMERS IS PREVENTED BY MUTATION BETA (Y341C)

被引：4
|
作者：
KAIBARA, C
ODAKA, M
HISABORI, T
YOSHIDA, M
机构：
[1] TOKYO INST TECHNOL, RESOURCES UTILIZAT RES LAB, R-1, NAGATSUTA 4259, YOKOHAMA, KANAGAWA 227, JAPAN
[2] TOKYO METROPOLITAN INST MED SCI, TOKYO 113, JAPAN
来源：
FEBS LETTERS | 1993年 / 321卷 / 01期
关键词：
F1-ATPASE; ALPHA-3-BETA-3 COMPLEX OF F1; ALPHA-1-BETA-1 HETERODIMER OF F1; ATP-INDUCED DISSOCIATION;
D O I：
10.1016/0014-5793(93)80618-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
AT(D)PMg induces dissociation of the alpha3beta3 complex of F1-ATPase from a thermophilic Bacillus strain, ps3, into the alpha1beta1 heterodimers [(1991) Biochim. Biophys. Acta 1056, 279-284] but the location of the AT(D)PMg binding site responsible is not known. From the analysis of AT(D)PMg binding properties of the isolated mutant beta subunit, beta(Y341C), and the stability of the alpha3beta(Y341C)3 complex in the presence of AT(D)PMg, we conclude tat binding of AT(D)PMg to the Tyr-341 site of the beta subunit(s) in the alpha3beta3 complex triggers the dissociation of the alpha3beta3 complex into the alpha1beta1, heterodimers.
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页码：46 / 50
页数：5
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