ISOELECTRIC-FOCUSING OF CASSAVA PROTOPLASTS

被引：3
|
作者：
SANTANA, MA [1 ]
VILLEGAS, L [1 ]
机构：
[1] INST INT ESTUDIOS AVANZADOS, UNIDAD BIOTECNOL, APARTADO 17606, CARACAS 1015A, VENEZUELA
来源：
PLANT PHYSIOLOGY | 1991年 / 95卷 / 01期
关键词：
D O I：
10.1104/pp.95.1.335
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Cassava (Manihot esculenta Crantz) protoplast was analyzed by using isoelectric focusing techniques. Two populations, representing 68 and 32% of the total sample, with mean isoelectric points of 4.48 and 4.60, were obtained using mesophyll protoplasts. The use of this technique allows demonstration of a discontinuous distribution of protoplast isoelectric point from one species according to their surface potential.
引用
收藏
页码：335 / 336
页数：2
相关论文
共 50 条
  • [1] SEPARATION OF LEAF PROTOPLASTS BY ISOELECTRIC-FOCUSING
    VILLEGAS, L
    BRAVATO, M
    [J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY, 1987, 23 (03): : A49 - A49
  • [2] THE POTENTIAL USE OF ISOELECTRIC-FOCUSING FOR SEPARATION OF POTATO PROTOPLASTS
    UIJTEWAAL, BA
    MOLENVELD, JW
    JOORDENS, GJMM
    [J]. POTATO RESEARCH, 1987, 30 (04) : 569 - 578
  • [3] ISOELECTRIC-FOCUSING
    GARFIN, DE
    [J]. METHODS IN ENZYMOLOGY, 1990, 182 : 459 - 477
  • [4] IMPROVEMENTS OF ISOELECTRIC-FOCUSING IN AGAROSE FOR DIRECT TISSUE ISOELECTRIC-FOCUSING
    THOMPSON, BJ
    DUNN, MJ
    BURGHES, AHM
    DUBOWITZ, V
    [J]. ELECTROPHORESIS, 1982, 3 (06) : 307 - 314
  • [5] METHODS OF ISOELECTRIC-FOCUSING
    BYLES, RH
    MCGUIRE, W
    SANDERS, MF
    [J]. AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, 1979, 50 (01) : 27 - 34
  • [6] ISOELECTRIC-FOCUSING OF PARVOVIRUSES
    SALO, RJ
    MAYOR, HD
    [J]. INTERVIROLOGY, 1978, 10 (02) : 87 - 93
  • [7] MINIREVIEW - ISOELECTRIC-FOCUSING
    PRATS, M
    [J]. BIOCHEMICAL EDUCATION, 1992, 20 (02): : 109 - 111
  • [8] ISOELECTRIC-FOCUSING OF RHODOPSIN
    ORLOV, NY
    FESENKO, EE
    KAZANTSEV, SI
    [J]. MOLECULAR BIOLOGY, 1976, 10 (05) : 923 - 930
  • [9] ISOELECTRIC-FOCUSING IN AGAROSE
    SARAVIS, CA
    ZAMCHECK, N
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1979, 29 (01) : 91 - 96
  • [10] ISOELECTRIC-FOCUSING AS A PUZZLE
    RIGHETTI, PG
    GIANAZZA, E
    BRENNA, O
    GALANTE, E
    [J]. JOURNAL OF CHROMATOGRAPHY, 1977, 137 (01): : 171 - 181
12345